Dobson Lab

You can get a full list from Pubmed. | ORCID 0000-0002-5506-4939. | Our Google Scholar page.

(2003-2022)

2022

  1. Turk LS, Currie MJ, Dobson RCJ, Comoletti D. (2022) Structure of Reelin repeat 8 and the adjacent C-terminal region. Biophys J., (in press)
  1. Bozzola T, Scalise M, Larsson C, Newton-Vesty M, Rovegno C, Mitra A, Cramer J, Wahlgren W, Santhakumari PR, Johnsson R, Schwardt O, Ernst B, Friemann R, Dobson RCJ, Indiveri C, Schelin J, Nilsson U, Ellervik U.(2022) Sialic acid derivatives inhibit SiaT transporters and delay bacterial growth. ACS Chem Biol., (in press)
  1. Harper AR, Dobson RCJ, Morris V, Moggré GJ (2022) Fermentation of plant-based dairy alternatives by lactic acid bacteria. Micro Biotech., 15 (5): 1404–21
  1. Meffan RC, Menges J, Dolamore F, Mak D, Fee C, Dobson RCJ, Nock V. (2022) Capillaric-Field Effect Transistors. Microsyst & Nanoeng., 8: 33
  1. Love MJ, Coombes D, Ismail S, Billington C, Dobson RCJ (2022) The structure and function of modular Escherichia coli O157:H7 bacteriophage FTBEc1 endolysin, LysT84: Defining a new endolysin catalytic subfamily. Biochem J., 479(2): 207–23
  1. Board AJ,  Crowther JM, Acevedo-Fani A, Meisrimler CN, Jameson GB, Dobson RCJ.(2022) How plants solubilise seed fats: revisiting oleosin structure and function to inform commercial applications. Biophys Rev.,  14: 257–66

2021

  1. Wood DM,  Dobson RCJ, Horne CR. (2021) Using Cryo-EM to uncover mechanisms of bacterial transcriptional regulation. Biochem Soc Trans., 49(6): 2711–26
  1. Turk LS, Kuang X, Pozzo VD, Patel K, Chen M, Huynh K, Currie MJ, Dobson RCJ, D’Arcangelo G, Dai W, Comoletti D. (2021) The structure-function relationship of a signaling competent, dimeric Reelin fragment. Structure, 29(10): 1156–70
  1. Arif T, Currie MJ, Dobson RCJ, Newson HL, Poonthiyil V, Fairbanks AJ, North RA, Rendle PM. (2021) Synthesis of N-acetylmannosamine-6-phosphate derivatives to investigate the mechanism of N-acetylmannosamine-6-phosphate 2-epimerase. Carbohydr Res., 510:  108445
  1. Lassé M, Stampfli AR, Orban T, Bothara RK, Gerrard JA, Fairbanks AJ, Pattinson NR, Dobson RCJ. (2021) Reaction dynamics and residue identification of haemoglobin modification by acrolein, a lipid-peroxidation by-product. Biochim Biophys Acta Gen Subj., 1865(12): 13001313
  1. Gilmour LH, Crowther JM, Porebski BT, Heath SG, Pattinson NR, Owen MC, Fredericks R, Buckle AM, Fee CJ, Göbl C, Dobson RCJ. (2021) Molecular basis of a redox switch: molecular dynamics simulations and surface plasmon resonance provide insight into reduced and oxidised angiotensinogen. Biochem J., 478(17): 3319-301
  1. Currie MJ, Manjunath L, Horne CR, Rendle PL, Subramanian R, Friemann R, Fairbanks AJ, Muscroft-Taylor AC, North RA, Dobson RCJ. (2021) N-Acetylmannosamine-6-phosphate 2-epimerase uses a novel substrate-assisted mechanism to catalyze amino sugar epimerization. J Biol Chem., 297(4): 10111
  1. Pilapitiya D, Harris P, Hanson-Manful P, McGregor R, Kowalczyk R, Raynes J, Carlton L, Dobson RCJ, Baker M, Brimble M, Lukomski S, Moreland N. (2021) Antibody responses to collagen peptides and Streptococcal collagen-like 1 proteins in acute rheumatic fever patients. Pathog Dis., 79(6): ftab033
  1. Love MJ, Coombes D, Manners SH, Abeysekera GS, Billington C, Dobson RCJ. (2020) Molecular basis for Escherichia coli O157:H7 phage FAHEc1 endolysin function and engineering increased thermal stability. Viruses, 13(6): 1101
  1. Davies JS, Currie MJ, Wright J, Newton-Vesty MC, North RA, Mace PD, Allison JR, Dobson RCJ. (2021) Selective nutrient transport in bacteria: multicomponent transporter systems reign supreme. Front Mol Biosci., 8: 578
  1. Horne CR, Venugopal H, Panjikar S, Wood DM, Henrickson A, E Brookes, North RA, Murphy JM, Friemann R, Griffin MDW, Ramm G, Demeler B, Dobson RCJ. (2021) Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism. Nat Commun., 12(1): 1–161
  1. Peskin AV, Meotti FC, Kean KM, Göbl C, Peixoto AS, Pace PE, Horne CR, Heath SG, Crowther JM, Dobson RCJ, Karplus PA, Winterbourn CC. (2021) Modifying the resolving cysteine affects the structure and hydrogen peroxide reactivity of peroxiredoxin 2. J Biol Chem., 296: 100494
  1. Parthasarathy A, Borrego EJ, Savka MA, Dobson RCJ, Hudson AO. (2021) Amino acid-derived secondary metabolites from plants: A potential source to facilitate novel antimicrobial development. J Biol Chem., 296: 100438
  1. Klionsky DJ, et al. (2021) Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy, 17(1): 1–382
  1. Menges J, Meffan C, Dolamore F, Fee C, Dobson RCJ, Nock V. (2021) New flow control systems in capillarics: Off valves. Lab Chip., 21: 205–14
  1. Manjunath L, Coombes D, Davies JD, Dhurandhar M, Dobson RCJ, Subramanian R, Bose S. (2021) Quaternary variations in the structural assembly of N-acetylglucosamine-6-phosphate deacetylase from Pasteurella multocida.  Proteins Struct Funct Genet., 89(1): 81–93

2020

  1. Dobson RCJ, Patel TR (2020) Analytical ultracentrifugation: still the gold standard that offers multiple solutions. Eur Biophys J., 49: 673–6
  1. Horne CR, Henrickson A, Demeler B, Dobson RCJ, (2020) Multi-wavelength analytical ultracentrifugation as a tool to characterise protein-DNA interactions in solution. Eur Biophys J. 49: 819–27
  1. Gangi Setty T, Sarkar A, Coombes DA, Dobson RCJ, R Subramanian (2020) Structure and Function of N-acetylmannosamine kinases from pathogenic bacteria. ACS Omega 5(48): 30923–36
  1. Love MJ, Dobson RCJ, Billington C (2020) Stemming the tide of antibiotic resistance by exploiting bacteriophages. The Biochemist 42(6): 6–11
  1. Crowther JM, Broadhurst M, Laue T, Jameson GB, Hodgkinson AG, Dobson RCJ. (2020) On the utility of fluorescence-detection analytical ultracentrifugation in probing biomolecular interactions in complex solutions: a case study in milk. Eur Biophys J. 49: 677–85
  1. Sugrue E, Coombes DA, Wood D, T Zhu, KA Donovan, Dobson RCJ (2020) The lid domain is important, but not essential, for catalysis of Escherichia coli pyruvate kinase. Eur Biophys J., 49: 761–72
  1. Suzuki H, Mahapatra D, Board AJ, Steel PJ, Dyer J, Gerrard JA, Dobson RCJ, Valéry C. (2020) Sub-Ångstrom structure of collagen model peptide (GPO)10 shows a hydrated triple helix with pitch variation and two proline ring conformations. Food Chem., 319: 126598
  1. Cree SL, Chua EW, Crowther JM, Dobson RCJ, Kennedy MA. (2020) G-quadruplex structures in amplicon DNA bind to EZ-Tn5 transposase. Biochimie 177: 190–7
  1. Weatherhead A, Crowther J, Horne C, Meng Y, Coombes D, Currie M, Watkin S, Adams L, Parthasarathy A, Dobson RCJ, Hudson AO. (2020) Structure-function studies of the antibiotic target L,L-diaminopimelate aminotransferase from Verrucomicrobium spinosum reveal an unusual oligomeric structure. Biochemistry 59(24): 2274–88
  1. Metcalfe RD, Aizel K, Zlatic CO, Nguyen PM, Morton CJ, Lio DSS, Cheng H-C, Dobson RCJ, Parker MW, Gooley PR, Putoczki TL, Griffin DW. (2020) The structure of the extracellular domains of human interleukin 11 α-receptor elucidates mechanisms of cytokine engagement. J Biol Chem., 295: 8285–301
  1. Horne CR, Kind L, Davies JS, Dobson RCJ (2020) Escherichia coli YjhC: an oxidoreductase involved in bacterial sialic acid metabolism.  Proteins: Struct. Funct. Genet., 88(5): 654–68
  1. Trevelyan SJ, Brewster JL, Burgess AE, Crowther JM, Cadell AL, Parker BJ, Croucher DR, Dobson RCJ, Murphy JM, Mace PD (2020) Structure-based mechanism of preferential complex formation by apoptosis signal–regulating kinases.  Sci Signal., 13(622): eaay6318
  1. Meng Y, Sheen CR, Magon NJ, Hampton MB, Dobson RCJ (2020) Structure-function analyses of alkylhydroperoxidase D from Streptococcus pneumoniae reveal an unusual three-cysteine active site architecture. J Biol Chem., 295: 2984–99
  1. Coombes D, Davies JS, Newton-Vesty M, Horne CR, TG Setty, Subramanian R, Moir JWB, Friemann R, Panjikar S, Griffin MDW, North RA, Dobson RCJ. (2020) The basis for non-canonical ROK family function in N-acetylmannosamine kinase from the pathogen Staphylococcus aureus. J Biol Chem., 295:3301–15.
  1. Nouchikian L, Lento C, Donovan KA, Dobson RCJ, Wilson D. (2020) Comparing the conformational stability of pyruvate kinase in the gas phase and in solution. J Am Soc Mass Spec., 31(3): 685–92
  1. Adams L, Rynkiewicz P, Babbitt GA, Mortensen JS, North RA, Dobson RCJ, Hudson AO. (2020) Comparative molecular dynamics simulations provide insight into antibiotic interactions: A case study using the enzyme L,L-diaminopimelate aminotransferase (DapL). Front Mol Biosci., 7: 46
  1. Love MJ, Abeysekera GS, Muscroft-Taylor AC, Billington C, Dobson RCJ. (2020) On the catalytic mechanism of bacteriophage endolysins: opportunities for engineering. BBA – Prot Proteom., 1868(1): 1403

2019

  1. Coombes D, Moir JWB, Poole AM, Cooper TF, Dobson RCJ. (2019) The fitness challenge of studying molecular adaptation. Biochem Soc Trans., 47(5): 1533–42
  1. Hoskin T, Crowther JM, Cheung J, Epton M, Sly P, Elder P, Dobson RCJ, Kettle A, Dickerhof N. (2019) Oxidative cross-linking of calprotectin occurs in vivo, altering its structure and susceptibility to proteolysis. Redox Biol., 24: 101202
  1. Crowther JM, Cross PJ, Oliver MR, Leeman MM, Bartl AJ, Weatherhead AW, North RA, Donovan KA, Griffin MDW, Suzuki H, Hudson AO, Kasanmascheff M, Dobson RCJ. (2019) Structure-function analyses of two plant meso-diaminopimelate decarboxylase isoforms reveal that active-site gating provides stereochemical control. J. Biol. Chem., 294(21): 8505–15
  1. Gilkes J, Sheen CR, Frampton RA, Smith GR, Dobson RCJ. (2019) The first purification of functional proteins from the unculturable, genome reduced, bottlenecked alpha-proteobacterium Candidatus Liberibacter solanacearum. Phytopathology, 109(7): 1141–8
  1. McKerchar H, Clerens S, Dobson RCJ, Maes E, Dyer J, Gerrard JA. (2019) Protein-protein crosslinking in food: characterisation methods, consequences and applications. Trends Food Chem. Tech., 86: 217–29
  1. Davies JS, Coombes D, Horne CR, Pearce FG, Friemann R, North RA, Dobson RCJ. (2019) Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus. FEBS Lett., 593(1): 52–66
  1. Mantravadi PK, Kalesh KA,  Dobson RCJ, Hudson AO, Parthasarathy A. (2019) The Quest for Novel Antimicrobial Compounds: Emerging Trends in Research, Development, and Technologies. Antibiotics, 8(1): 8

2018

  1. Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Muñoz-Lintz DA, Dobson RCJ, Remus DM, Remus-Emsermann MNP.
    (2018) Chromatic bacteria – a broad host-range plasmid and chromosomal insertion toolbox for fluorescent protein expression in bacteria. Front Microbiol., 9: 3052
  1. Foglizzo M, Middleton AJ, Burgess AE, Crowther JM, Dobson RCJ, Murphy JM, Day CL, Mace PD. (2018) A bidentate Polycomb Repressive-Deubiquitinase complex is required for efficient activity on nucleosomes. Nat Comm., 9(1): 3932
  1. Manjunath L, Guntupalli SR, Currie MJ, North RA, Dobson RCJ, Nayak V, Subramanian R. (2018) Crystal structures and kinetic analyses of N-acetylmannosamine-6-phosphate 2-epimerases from Fusobacterium nucleatum and Vibrio cholerae. Acta Cryst F Struct. Biol Comm., 74(7): 431–40
  1. North RA, Wahlgren WY, Remus D, Scalise M, Kessans SA, Dunevall E, Claesson E, Soares da Costa T, Perugini MA, Subramanian R, Allison JM, Indiveri C, Friemann R, Dobson RCJ. (2018) The sodium sialic acid symporter from Staphylococcus aureus has altered substrate specificity. Front Chem., 6: 233
  1. Wahlgren WY, Dunevall E, North RA, Paz A, Scalise M, Bisignano P, Bengtsson-Palme J, Goyal P, Claesson E, Caing-Carlsson R, Andersson R, Beis K, Nilsson UJ, Farewell A, Pochini L, Indiveri C, Grabe M, Dobson RCJ, Abramson J, Ramaswamy S, Friemann R. (2018) Substrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site. Nat Comm., 9(1): 1753
  1. Crowther JM, Allison JR, Smolenski GA, Hodgkinson AJ, Jameson GB, Dobson RCJ. (2018) The self-association and thermal denaturation of caprine and bovine β-lactoglobulin. Euro Biophys J., 16(6): 961
  1. Love MJ, Bhandari D, Dobson RCJ, Billington C. (2018) Potential for bacteriophage endolysins to supplement or replace antibiotics in food production and clinical care. Antibiotics, 7(1): 17
  1. Parthasarathy A, Cross PJ, Dobson RCJ, Adams LE, Savka MA, Hudson AO. (2018) A three-ring circus: Metabolism of the three proteogenic aromatic amino acids and their role in the health of plants and animals. Front Mol Biosci., 5: 303–30
  1. Gilkes JM, Frampton RA, Smith GR, Dobson RCJ. (2018) Potential pathogenicity determinants in the genome of ‘Candidatus Liberibacter solanacearum’, the causal agent of zebra chip disease of potato. Aust Plant Path., 47(2): 119–34
  1. Atkinson SC, Dogovski C, Wood L, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA. (2018) Substrate locking promotes dimer-dimer docking of an enzyme antibiotic target. Structure, 26(7): 948–59
  1. Peng F, Widmann S, Wünsche A, Duan K, Donovan KA, Dobson RCJ, Lenski RE, Cooper TF. (2018) Effects of beneficial mutations in pykF gene vary over time and across replicate populations in a long-term experiment with bacteria. Mol Biol Evol., 35(1): 202-10

2017

  1.  North RA, Horne CR, Davies JS, Remus DM, Muscroft-Taylor AC, Goyal P, Wahlgren WY, Ramaswamy S, Friemann R, Dobson RCJ. (2017) “Just a spoonful of sugar…”: import of sialic acid across bacterial cell membranes. J Biophys Rev., 10(2): 219–27
  1. Kavianinia I, Yang SH, Kaur H, Harris PWR, Dobson RCJ, Fairbanks AJ, Brimble MA (2017) Synthesis and incorporation of an advanced lipid peroxidation end-product building block into collagen mimetic peptides. Chem Comm., 53: 8459–62
  1. Rogov VV, Stolz A, Ravicahandran AC, Rios-Szwed DO, Suzuki H, Kniss A, Löhr F, Wakatsuki S, Dötsch V, Dikic I*, Dobson RCJ*, McEwan DG* (2017) Structural and Functional analysis of the GABARAP Interaction Motif (GIM). EMBO R., 18(8): 1382–96
    * corresponding author
  1. Pearce FG, Hudson AO, Loomes K, Dobson RCJ. (2017) Dihydrodipicolinate synthase: Structure, dynamics, function, and evolution. Subcell Biochem., 83: 271–89.

2016

  1. North RA, Watson AJA, Pearce FG, Muscroft-Taylor AC, Friemann R, Fairbanks AJ, Dobson RCJ. (2016) Structure and inhibition of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus. FEBS Letters, 590(23): 4414–28. Link
  1. Cala AR, Nadeau MT, Staker BL, Weatherhead AW, Dobson RCJ, Abendroth J, Myler PJ, Hudson AO. (2016) The crystal structure of dihydrodipicolinate reductase at 2.3 Å resolution from the human pathogenic bacterium Bartonella henselae str. Houston-1. Acta Crystallogr F Struct Biol Commun., 72(12): 885–91.
  1. Umu SU, Poole AM, Dobson RCJ, Gardner PP (2016) Avoidance of stochastic RNA interactions can be harnessed to control protein expression levels in bacteria and archaea. eLife, 5: e13479. Link
  1. Lee J, Joshi N, Pasini R, Dobson RCJ, Allison JA, Leustek T. (2016) Inhibition of Arabidopsis growth by the allelopathic compound azetadine 2-carboxylate is due to the low amino acid specificity of cytosolic prolyl-tRNA synthetase. Plant J., 88(2): 236–46.
  1. Chen K, Dobson RCJ, Lucet IS, Young SN, Pearce FG, Blewitt ME, Murphy MJ. (2016) The epigenetic regulator Smchd1 contains a functional GHKL-type ATPase domain. Biochem J., 473(12): 1733–44. Link
  1. Naqvi KF, Patin D, Wheatley MS, Savka MA, Dobson RCJ, Gan HM, Barreteau H, Blanot D, Mengin-Lecreul D, Hudson AO. (2016) Identification and partial characterization of a novel UDP-N-acetylenolpyruvoylglucosamine reductase/UDP-N-acetylmuramate:L-alanine ligase fusion enzyme from Verrucomicrobium spinosum DSM 4136T. Front Microbiol.,. 7: 362. Link
  1. Poen S, Nakatan Ybic1621-16-cover.inddi, Opel-Reading HK, Lassé M, Dobson RCJ, Krause KL. (2016) Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery. Biochem J., 473(9): 1267–80. Link
  1. Donovan KA, Zhu S, Liuni P, Peng F, Kessans SA, Wilson DA, Dobson RCJ. (2016) Conformational dynamics and allostery in pyruvate kinase. J Biol Chem., 291(17): 9244–56. Link
  1. Screen Shot 2016-05-14 at 7.43.15 PM Donovan KA, Atkinson SC, Kessans SA, Peng F, Cooper TF, Griffin MDW, Jameson GB, Dobson RCJ, (2016) Grappling with anisotropic data, pseudo-merohedral twinning and pseudo-translational non-crystallographic symmetry: A case study involving pyruvate kinase. Acta Crystallogr D Biol Crystallogr., 72(4): 512–9. Link
  1. Naqvi KF, Staker BL, Dobson RCJ, Serbzhinskiy D, Sankaran B, Myler PJ, Hudson AO. (2016) Cloning, expression, purification, crystallization and X-ray diffraction analysis of dihydrodipicolinate synthase from the human pathogenic bacterium Bartonella henselae strain Houston-1 at 2.1 Å resolution. Acta Crystallogr F Struct Biol Commun., 72,(1): 2–9. Link
  1. Klionsky DJ, et al. (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1) 1–222. Link

2015

  1. Chen K, Hu J, Moore DL, Liu R, Kessans SA, Breslin K, Lucet IS, Keniry A, Leong HS, Parish CL, Hilton DJ, Lemmers RJLF, van der Maarel SM, Czabotar PE, Dobson RCJ, Ritchie ME, Kay GF, Murphy JM, Blewitt ME. (2015) Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation. Proc Natl Acad Sci USA, 112(27): E3535–44. Link

2014

  1. Screen Shot 2015-10-11 at 10.27.03 AMCrowther JM, Lassé M, Suzuki H, Kessans SA, Loo TS, Norris GE, Hodgkinson AJ, Jameson GB, Dobson RCJ. (2014) Ultra-high resolution crystal structure of recombinant caprine β-lactoglobulin. FEBS Lett., 588(21): 3816–22. Link 
  1. Triassi AJ, Wheatley MS, Savka MA, Gan HM, Dobson RCJ, Hudson AO (2014) L,L-Diaminopimelate aminotransferase (DapL): a putative target for the development of narrow-spectrum antibacterial compounds. Front Microbial., 5: 509 Link
  1. McKinnie SKM, Rodriguez-Lopez EM, Vederas JC, Crowther JM, Suzuki H, Dobson RCJ, Leustek T, Triassi AJ, Wheatley MS, Hudson AO. (2014) Differential response of orthologous L,L-diaminopimelate aminotransferases (DapL) to enzyme inhibitory antibiotic lead compounds. Bioorg Med Chem., 22(1): 523–30. Link
  1. stfode 22_1.c2.indd Suzuki H*, Tabata T, Morita E, Kawasaki M, Kato R, Dobson RCJ*, Yoshimori T, Wakatsuki S. (2014) Structural basis of the autophagy-related LC3/Atg13 LIR complex: recognition and interaction mechanism. Structure, 22(1): 47–58. Link
    * corresponding author.
    This work was the subject of an invited commentary: “A small switch has a large effect on autophagy” Winfried Weissenhorn, Marie-Odile Fauvarque. Link
  1. Nazmi AR, Schofield LR, Dobson RCJ, Jameson GB, Parker EJ. (2014) Destabilization of the homotetrameric assembly of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from the hyperthermophile Pyrococcus furiosus enhances enzymatic activity. J Mol Biol., 426(3): 656–73. Link
  1. North RA, Seizova S, Stampfli A, Kessans SA, Suzuki H, Griffin MDW, Kvansakul M, Dobson RCJ. (2014) Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of N-acetylmannosamine kinase from methicillin-resistant Staphylococcus aureus. Acta Crystallogr F Struct Biol Commun., 70(5): 643–9. Link
  1. Oliver MR, Crowther MJ, Leeman MM, Kessans SA, North RA, Donovan KA, Griffin MDW, Suzuki H, Hudson AO, Kasanmascheff M, Dobson RCJ. (2014) The purification, crystallization and preliminary X-ray diffraction analysis of two isoforms of meso-diaminopimelate decarboxylase from Arabidopsis thaliana. Acta Crystallogr F Struct Biol Commun., 70(5): 663–8. Link
  1. North RA, Kessans SA, Griffin MDW, Watson AJA, Fairbanks AJ, Dobson RCJ (2014) Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of N-acetylmannosamine-6-phosphate 2-epimerase from methicillin-resistant Staphylococcus aureus. Acta Crystallogr F Struct Biol Commun., 70(5): 650–5. Link
  1. Atkinson SC, Hor L, Dogovski C, Dobson RCJ, Perugini MA. (2014) Identification of the bona fide DHDPS from a common plant pathogen. Proteins, 82(9): 1869–883. Link
  1. Putoczki TL, Dobson RCJ, Griffin MDW. (2014) The structure of human interleukin-11 reveals receptor-binding site features and structural differences from interleukin-6. Acta Crystallogr D Biol Crystallogr., 70(9): 2277–85. Link
  1. Hildebrand JM, Tanzer MC, Lucet IS, Young SN, Spall SK, Sharma P, Pierotti C, Garnier J-M, Dobson RCJ, Webb AI, Tripaydonis A, Babon JJ, Mulcair MD, Scanlon MJ, Alexander WS, Wilks AF, Czabotar PE, Lessene G, Murphy JM, Silke J. (2014) Activation of the pseudokinase MLKL unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death. Proc Natl Acad Sci U.S.A., 111(42): 15072–77.
    Link

2013

  1. Sikorski A, Argüello-Astorga GR, Dayaram A, Dobson RCJ, Varsani A. (2013) Discovery of a novel circular single-stranded DNA virus from porcine faeces. Arch Virol., 158(1): 283–9. Link
  1. Murphy J, Czabotar P, Hildebrand J, Lucet I, Zhang J, Alvarez-Diaz S, Lewis R, Lalaoui N, Metcalf D, Young S, Varghese L, Tannahill G, Hatchell E, Majewski I, Okamoto T, Dobson RCJ, et al. (2013) Regulation of necroptosis by the pseudokinase Mixed Lineage Kinase domain-Like. Immunity 39(3):443–53
  1. Atkinson SC, Dogovski C, Downton MT, Czabotar PE, Dobson RCJ, Gerrard JA, Wagner J, Peruguini MA. (2013) Structural, kinetic and computational investigation of Vitis vinifera DHDPS reveals new insight into the mechanism of lysine-mediated allosteric inhibition. Plant Mol Biol., 81(4): 431–46. Link
  1. North RA, Kessans SA, Atkinson SC, Suzuki H, Watson AJA, Burgess BR, Angley LM, Hudson AO, Varsani A, Griffin MDW, Fairbanks AJ, Dobson RCJ. (2013) Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus. Acta Crystallogr Sect F Struct Biol Cryst Commun., 69(3): 306–12. Link
  1. Cross PJ, Allison TM, Dobson RCJ, Jameson GB, Parker EJ. (2013) Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain. Proc Natl Acad Sci U.S.A., 110(6): 2111–6. Link
  1. Hor L, Dobson RCJ, Downton MT, Wagner J, Hutton CA, Perugini MA (2013) Dimerization of bacterial diaminopimelate epimerase is essential for catalysis. J Biol Chem., 288(13): 9238–48. Link
  1. McGroty SE, Pattaniyil TD, Patin D, Blanot D, Ravichandran AC, Suzuki H, Dobson RCJ, Savka MA, Hudson AO. (2013) Biochemical characterization of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate: meso-2,6-diaminopimelate ligase (MurE) from Verrucomicrobium spinosum DSM 4136T. PLoS ONE, 8(6): e66458–9. Link

2012

  1. Griffin MDW, Billakanti JM, Wason A, Keller S, Mertens HDT, Atkinson SC, Dobson RCJ, Perugini MA, Gerrard JA, Pearce FG (2013) Characterisation of the first enzymes committed to lysine biosynthesis in Arabidopsis thaliana. PLoS ONE, 7(7): e40318. Link
  1. Nachar VR, Savka FC, McGroty SE, Donovan KA, North RA, Dobson RCJ, Buckley LJ, Hudson AO. (2012) Genomic and biochemical analysis of the diaminopimelate and lysine biosynthesis pathway in Verrucomicrobium spinosum: Identification and partial characterization of L,L-diaminopimelate aminotransferase and UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-meso-diaminopimelate ligase. Front Microbial., 3: 183. Link
  1. Dayaram A, Opong A, Jäschke A, Hadfield J, Baschiera M, Dobson RCJ, Offei KS, Shepherd DN, Martin DP, Varsani A. (2012) Molecular characterisation of a novel cassava associated circular ssDNA virus, Virus Res., 166(1): 130–5. Link
  1. Reboul CF, Porebski BT, Griffin MDW, Dobson RCJ, Perugini MA, Gerrard JA, Buckle AM. (2012) Structural and dynamic requirements for optimal activity of the essential bacterial enzyme dihydrodipicolinate synthase. PLoS Comp Biol., 8(6): e1002537–12. Link
  1. Atkinson SC, Dogovski C, Downton MT, Pearce FG, Reboul CF, Buckle AM, Gerrard JA, Dobson RCJ, Wagner J, Perugini MA. (2012) Crystal, Solution and in silica structural studies of dihydrodipicolinate synthase from the common grapevine. PLoS ONE, 7(6): e38318–9. Link
  1. bpj103_2.c1.indd Mercadante D, Melton LD, Norris GE, Loo TS, Williams MAK, Dobson RCJ, Jameson GB. (2012) Bovine β-lactoglobulin is dimeric under imitative physiological conditions: dissociation equilibrium and rate constants over the pH range of 2.5–7.5. Biophys J., 103(2): 303–12. Link
  1. Boughton BA, Dobson RCJ, Hutton CA. (2012) The crystal structure of dihydrodipicolinate synthase from Escherichia coli with bound pyruvate and succinic acid semialdehyde: unambiguous resolution of the stereochemistry of the condensation product. Proteins, 80(8): 2117–22. Link
  1. Atkinson SC, Dogovski C, Dobson RCJ, Perugini MA. (2012) Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from Agrobacterium tumefaciens. Acta Crystallogr Sect F Struct Biol Cryst Commun., 68(9): 1040–47. Link

2011

  1. Hudson AO†, Girón I, Dobson RCJ†. (2011) Crystallization and preliminary X-ray diffraction analysis of L,L-diaminopimelate aminotransferase (DapL) from Chlamydomonas reinhardtii. Acta Crystallogr Sect F Struct Biol Cryst Commun., 67(1): 140–3. Link
    † = Corresponding author
  1. Veldhuis NA, Kuiper MJ, Dobson RCJ, Pearson RB, Camakaris J. (2011) In silico modeling of the Menkes copper-translocating P-type ATPase 3rd metal binding domain predicts that phosphorylation regulates copper-binding. Biometals, 24(3): 477–87. Link
  1. Gunn N, Gorman MA, Dobson RCJ, Parker MW, Mulhern TD (2011) Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase. Acta Crystallogr Sect F Struct Biol Cryst Commun., 67(3): 336–9. Link
  1. Cross PJ, Dobson RCJ, Patchett ML, Parker EJ. (2011) Tyrosine latching of a regulatory gate affords allosteric control of aromatic amino acid biosynthesis. J Biol Chem., 286(12): 10216–24. Link
  1. Dobson RCJ†, Girón I, Hudson AO†, (2011) L,L-Diaminopimelate aminotransferase from Chlamydomonas reinhardtii: A Target for algaecide development. PLoS ONE, 6(5): e20439–13. Link
    † = Corresponding author
  1. Evans G, Schuldt L, Griffin MDW, Devenish SRA, Pearce FG, Perugini MA, Dobson RCJ, Jameson GB, Weiss MS, Gerrard JA. (2011) A tetrameric structure is not essential for activity in dihydrodipicolinate synthase (DHDPS) from Mycobacterium tuberculosis. Arch Biochem Biophys., 512(2): 154–9. Link
  1. Hudson AO, Klartag A, Gilvarg C, Dobson RCJ, Marques FG, Leustek T. (2011) Dual diaminopimelate biosynthesis pathways in Bacteroides fragilis and Clostridium thermocellum. Biochim Biophys Acta, 1814(9): 1162–8. Link
  1. Griffin MDW, Billakanti JM, Gerrard JA, Dobson RCJ, Pearce FG. (2011) Crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase 2 from Arabidopsis thaliana. Acta Crystallogr Sect F Struct Biol Cryst Commun., 67(11): 1386–90. Link
  1. Pearce FG, Dobson RCJ, Jameson GB, Perugini MA, Gerrard JA. (2011) Characterization of monomeric dihydrodipicolinate synthase variant reveals the importance of substrate binding in optimising oligomerization. BBA – Proteins and Proteomics, 1814(12): 1900–9. Link
  1. Atkinson SC, Dogovski C, Newman J, Dobson RCJ, Perugini MA. (2011) Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine Vitis vinifera. Acta Crystallogr Sect F Struct Biol Cryst Commun., 67(12): 1537–41. Link

2010

  1. Dommaraju S, Gorman MA, Dogovski C, Pearce FG, Gerrard JA, Dobson RCJ, Parker MW, Perugini MA. (2010) Cloning, expression and crystallization of dihydrodipicolinate reductase from methicillin-resistant Staphylococcus aureus. Acta Crystallogr Sect F Struct Biol Cryst Commun., 66(1): 57–60.
  1. Zhu T, Bailey MF, Angley LM, Cooper TF, Dobson RCJ. (2010) The quaternary structure of pyruvate kinase type 1 from Escherichia coli at low nanomolar concentrations. Biochimie, 92(1): 116–20. Link
  1. Hor L&, Dobson RCJ&, Dogovski C, Hutton CA, Perugini MA (2010) Crystallization and preliminary X-ray diffraction analysis of diaminopimelate epimerase from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun., 66(1): 37–40. Link
    & = Joint first author
  1. ABB_coverGriffin MDW, Dobson RCD, Gerrard JA, Perugini MA. (2010) Exploring the dihydrodipicolinate synthase tetramer: How resilient is the dimer–dimer interface? Arch Biochem Biophys., 494(1): 58–63. Link
  1. Voss JE, Scally SW, Taylor NL, Atkinson SC, Griffin MDW, Hutton CA, Parker MW, Alderton MR, Gerrard JA, Dobson RCJ, Dogovski C, Perugini MA. (2010) Substrate-mediated stabilization of a tetrameric drug target reveals Achilles heel in anthrax. J Biol Chem., 285(8): 5188–95. Link
  1. Soares da Costa TP, Muscroft-Taylor AC, Dobson RCJ, Devenish SRA, Jameson GB, Gerrard JA. (2010) How essential is the ‘essential’ active-site lysine in dihydrodipicolinate synthase? Biochimie, 92(7): 837–45. Link
  1. Wubben JM, Dogovski C, Dobson RCJ, Codd R, Gerrard JA, Parker MW, Perugini MA. (2010) Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the psychrophile Shewanella benthica. Acta Crystallogr Sect F Struct Biol Cryst Commun., 66(11): 1511–16. Link
  1. Muscroft-Taylor AC, Catchpole RJ, Dobson RCJ, Pearce FG, Perugini MA, Gerrard JA. (2010) Disruption of quaternary structure in Escherichia coli dihydrodipicolinate synthase (DHDPS) generates a functional monomer that is no longer inhibited by lysine. Arch Biochem Biophys., 503(2): 202–06. Link
  1. Chan K-C, Lio DS-S, Dobson RCJ, Jarasrassamee B, Hossain MI, Roslee AK, Ia KK, Perugini MA, Cheng H-C (2010) Development of the procedures for high-yield expression and rapid purification of active recombinant Csk-homologous kinase (CHK): Comparison of the catalytic activities of CHK and CSK. Prot Express Purif., 74(2): 139–47. Link

2009

  1. Domigan LJ, Scally SW, Fogg MJ, Hutton CA, Perugini MA, Dobson RCJ, Muscroft-Taylor AC, Gerrard JA, Devenish SRA. (2009) Characterisation of dihydrodipicolinate synthase (DHDPS) from Bacillus anthracis. Biochim Biophys. Acta, 1794(10): 1510–16. Link
  1. James PF&, Dogovski C&, Dobson RCJ&, Bailey MF, Goldie KN, Karas JA, Scanlon DB, O’Hair JRA, Perugini MA. (2009) Aromatic residues in the C-terminal helix of human apoC-I mediate phospholipid interactions and particle morphology. J Lipid Res., 50(7): 1384–94. Link
    & = Joint first author.
  1. Guo BBB, Devenish SRA, Dobson RCJ, Muscroft-Taylor AC, Gerrard JA. (2009) The C-terminal domain of Escherichia coli dihydrodipicolinate synthase (DHDPS) is essential for maintenance of quaternary structure and efficient catalysis. Biochem Biophys Res Commun., 380(4): 802–6. Link
  1. Atkinson SC&, Dobson RCJ&,†, Newman JM, Gorman MA, Dogovski C, Parker MW, Perugini MA. (2009) Crystallization and preliminary X-ray analysis of dihydrodipicolinate synthase from Clostridium botulinum in the presence of its substrate pyruvate. Acta Crystallogr Sect F Struct Biol Cryst Commun., 65(3): 253–5. Link
    & = Joint first author † = Corresponding author
  1. Voss JE, Scally SW, Taylor NL, Dogovski C, Alderton MR, Hutton CA, Gerrard JA, Parker MW, Dobson RCJ†, Perugini MA† (2009) Expression, purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Bacillus anthracis in the presence of pyruvate. Acta Crystallogr Sect F Struct Biol Cryst Commun., 65(2): 188–91. Link
    † = Corresponding author
  1. Dobson RCJ†, Perugini MA, Jameson GB, Gerrard JA (2009) Specificity versus catalytic potency: The role of threonine 44 in Escherichia coli dihydrodipicolinate synthase mediated catalysis. Biochimie, 91(8): 1036–44. Link
    † = Corresponding author

2008

  1. Boughton BA, Griffin MDW, O’Donnell PA, Dobson RCJ, Perugini MA, Gerrard JA, Hutton CA (2008) Irreversible inhibition of dihydrodipicolinate synthase by 4-oxo-heptenedioic acid analogues. Bioorg Med Chem., 16(23): 9975–83. Link
  1. Boughton BA, Dobson RCJ, Gerrard JA, Hutton CA. (2008) Conformationally constrained diketopimelic acid analogues as inhibitors of dihydrodipicolinate synthase. Bioorg Med Chem Lett., 18(2): 460–3. Link
  1. Mitsakos V, Dobson RCJ, Pearce FG, Devenish SRA, Evans GL, Burgess BR, Perugini MA, Gerrard JA, Hutton CA (2008) Inhibiting dihydrodipicolinate synthase across species: towards specificity for pathogens? Bioorg Med Chem Lett., 18(2): 842–4. Link
  1. Dobson RCJ†, Atkinson SC, Gorman MA, Newman JM, Parker MW, Perugini MA. (2008) The purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Clostridium botulinum. Acta Crystallogr Sect F Struct Biol Cryst Commun., 64(3): 206–8. Link
    † = Corresponding author
  1. Kefala G, Evans GL, Griffin MDW, Devenish SRA, Pearce FG, Perugini MA, Gerrard JA, Weiss MS†, Dobson RCJ†. (2008) Crystal structure and kinetic study of dihydrodipicolinate synthase from Mycobacterium tuberculosis. Biochem J., 411(2): 351–60. Link
    † = Corresponding author
  1. Androulakis S, Schmidberger J, Bate MA, DeGori R, Beitz A, Keong C, Cameron B, McGowan S, Porter CJ, Harrison A, Hunter J, Martin J, Kobe B, Dobson RCJ, Parker MW, Whisstock JC, Gray J, Treloar A, Groenewegen D, Dickson D, Buckle AM. (2008) Federated repositories of X-ray diffraction images. Acta Crystallogr D Biol Crystallogr., 64(7): 810–4. Link
  1. Burgess BR, Dobson RCJ†, Dogovski C, Jameson GB, Parker MW, Peruguini MA†. (2008) Purification, crystallization and preliminary X-ray diffraction studies to near-atomic resolution of dihydrodipicolinate synthase from methicillin-resistant Staphylococcus aureus. Acta Crystallogr Sect F Struct Biol Cryst Commun., 64(7): 659–61. Link
    † = Corresponding author
  1. Griffin MDW&, Dobson RCJ&, Pearce FG, Antonio L, Whitten AE, Liew CK, Mackay JP, Trewhella J, Jameson GB, Perugini MA, Gerrard JA. (2008) Evolution of quaternary structure in a homotetrameric enzyme. J Mol Biol., 380(4): 691–703. Link
    & = Joint first author.
  1. Burgess BR&, Dobson RCJ&, Bailey MF, Atkinson SC, Griffin MDW, Jameson GB, Parker MW, Gerrard JA, Perugini MA (2008) Structure and evolution of a novel dimeric enzyme from a clinically important bacterial pathogen. J Biol Chem., 283(41): 27598–603. Link
    & = Joint first author.
  1. Pearce FG, Dobson RCJ, Weber A, Lane LA, McCammon MG, Squire MA, Perugini MA, Jameson GB, Robinson CV, Gerrard JA. (2008) Mutating the tight-dimer interface of dihydrodipicolinate synthase disrupts the enzyme quaternary structure: Toward a monomeric enzyme. Biochemistry, 47(46): 12108–17. Link
  1. Dobson RCJ†, Griffin MDW, Devenish SRA, Pearce FG, Hutton CA, Gerrard JA, Jameson GB, Perugini MA. (2008) Conserved main-chain peptide distortions: a proposed role for Ile203 in catalysis by dihydrodipicolinate synthase. Protein Sci., 17(12): 2080–90. Link
    † = Corresponding author
  1. Devenish SRA, Gerrard JA, Jameson GB, Dobson RCJ (2008) The high-resolution structure of dihydrodipicolinate synthase from Escherichia coli bound to its first substrate, pyruvate. Acta Crystallogr Sect F Struct Biol Cryst Commun., 64(12): 1092–5. Link

2005

  1. Turner JJ, Healy JP, Dobson RCJ, Gerrard JA, Hutton CA. (2005) Two new irreversible inhibitors of dihydrodipicolinate synthase: diethyl (E,E)-4-oxo-2,5-heptadienedioate and diethyl (E)-4-oxo-2-heptenedioate. Bioorg Med Chem Lett., 15(4): 995–8. Link
  1. Dobson RCJ, Griffin MDW, Jameson GB, Gerrard JA. (2005) The crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from Escherichia coli with improved resolution show new features of biological significance. Acta Crystallogr D Biol Crystallogr., vol. 61, no. 8, pp. 1116–24. Link
  1. Screen Shot 2015-10-16 at 10.13.20 PMDobson RCJ, Devenish SRA, Turner LA, Clifford VR, Pearce FG, Jameson GB, Gerrard JA. (2005) Role of arginine 138 in the catalysis and regulation of Escherichia coli dihydrodipicolinate synthase. Biochemistry, 44(39): 13007–13. Link

2004

  1. Roberts SJ, Morris JC, Dobson RCJ, Baxter CL, Gerrard JA. (2004) Complete syntheses of (S)-aspartate semi-aldehyde and demonstration that Δ2-tetrahydroisophthalic acid is a non-competitive inhibitor of dihydrodipicolinate synthase. Arkivoc, x: 166-77. Link
  1. Dobson RCJ, J. A. Gerrard and F. G. Pearce (2004) Dihydrodipicolinate synthase is not inhibited by its substrate, (S)-aspartate β-semialdehyde. Biochem J., 377(3): 757–62. Link
  1. Dobson RCJ, Griffin MDW, Roberts SJ, Gerrard JA. (2004) Dihydrodipicolinate synthase (DHDPS) from Escherichia coli displays partial mixed inhibition with respect to its first substrate, pyruvate. Biochimie, 86(4): 311–5. Link
  1. Screen Shot 2015-10-16 at 10.05.33 PMDobson RCJ, Valegård K, Gerrard JA (2004) The crystal structure of three site-directed mutants of Escherichia coli dihydrodipicolinate synthase: Further evidence for a catalytic triad. J Mol Biol., 338(2): 329–39. Link

2003

  1. Roberts SJ, Morris JC, Dobson RCJ, Gerrard JA. (2003) The preparation of (S)-aspartate semi-aldehyde appropriate for use in biochemical studies. Bioorg Med Chem Lett., 13(2): 265–7. Link

Book Chapters

  1. Watkin SAJ, Ryan TM, Miller AJ, Nock VM, Pearce FG, Dobson RCJ (2017) Microfluidics for small-angle X-ray scattering. in X-ray Scattering, (A. E. Ares, eds.), Rijeka: InTech Chapter 4, pp. 89–104.
  1. Hudson AO, Savka MA, Pearce FG, Dobson RCJ. (2017) Lysine biosynthesis in microorganisms. in Handbook of Microbial Metabolism of Amino Acids, Chapter 4, pp. 49–69.
  1. Crowther JM, Jameson GB, Hodgkinson AJ, Dobson RCJ. (2016) Structure, oligomerisation and interactions of β-lactoglobulin. in Milk Proteins – from structure to biological properties and health aspects, Edited by Isabel Gigli, ISBN 978-953-51-4584-4, pp. 33–50.
  1. Dogovski C, Atkinson SC, Dommaraju SR, Dobson RCJ, Perugini MA. (2009) Lysine biosynthesis in bacteria: an unchartered pathway for novel antibiotic design. in Encyclopedia of life support systems, 11: 116–36.

Published conference proceedings (peer-reviewed, presenter)

  1. Meffan C, Menges J, Mak D, Dolamore F, Fee C, Dobson RCJ, Nock V (2022) Field effect transistor-like control of capillaric flow using off valves.  35th International Conference on Micro Electro Mechanical Systems. Tokyo, Japan (Talk)
  1. Meffan C, Menges J, Dolamore F, Fee C, Dobson RCJ, Nock V (2020) Transistor OFF-valve based feedback, metering and logic operations in capillary microfluidics.  34th International Conference on Micro Electro Mechanical Systems. Gainesville, FL, USA (Talk)
  1. Mandani A, Thompson D., Dobson RCJ, Gieseg S, Badami K. (2013) Electro-Chemical Changes with ABO-Incompatible Red Blood Cell Transfusions. Gold Coast, Australia: HAA 2013, 20-23 Oct 2013.  (Poster)
  1. Giron IO, Dobson RCJ, Hudson AO. (2011) Characterization of L,L-diaminopimelate aminotransferase from Chlamydomonas reinhardtii. Minneapolis, WI, USA: Plant Biology 2011, 6-10 Aug 2011. (Poster)
  1. Zhu T, Fei N, Perugini MA, Cooper TF, Dobson RCJ. (2008) In vivo evolution of Escherichia coli pyruvate kinase type I: How does genotypic evolution affect phenotype?  J Proteom Bioinform., 2, 237-8 (Poster)
  1. Griffin MDW, Angley LM, Webb L, Dobson RCJ, Jameson GB, Gerrard JA, Perugini MA. (2006) Proteomics at the quaternary level: Insight into novel antibiotic design. Minneapolis, Asia Pacific Journal of Clinical Oncology, 2: A53 (Talk)

Popular press

  1. Battle of the Bugs, Listener article, 2021. Issue 39, describes our work with ESR discovering new antibiotics. (Press article)
  1. Stemming the tide of antibiotic resistance by exploiting bacteriophages, 2020 The Biochemist 42(6):6 (popular science article, also Journal article #119 above)
  1. Mills N (2009) Profile of a killer enzyme. Australasian Science March issue (Press article)
  1. Jones K (2007/8) Protein-protein interactions and enzymology laboratory. What’s New in Lab Technology. 18(5): 8. (Press article)
  1. Hannink N (2008) Cocktail to fix bugs that fight drugs. The Voice 3(9): 6 (Press article)

Thesis

  1. Dobson RCJ (2004) Investigating the catalytic and regulatory mechanisms of dihydrodipicolinate synthase. PhD, University of Canterbury, Christchurch, New Zealand.